Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture Part 2: Image sequence analysis based evaluation and biological application
  • Metaadatok
Tartalom: http://real.mtak.hu/58583/
Archívum: MTA Könyvtár
Gyűjtemény: Status = In Press

Type = Article
Cím:
Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture Part 2: Image sequence analysis based evaluation and biological application
Létrehozó:
Járvás, Gabor
Varga, Tamas.
Szigeti, Marton G.
Hajba, Laszlo
FĂĽrjes, Peter
Rajta, István
Guttman, Andras
Kiadó:
Wiley-VCH Verlag
Dátum:
2017
Téma:
QD01 Analytical chemistry / analitikai kémia
Tartalmi leírás:
As a continuation of our previously published work, this paper presents a detailed evaluation of a microfabricated cell capture device utilizing a doubly tilted micropillar array. The device was fabricated using a novel hybrid technology based on the combination of proton beam writing and conventional lithography techniques. Tilted pillars offer unique flow characteristics and support enhanced fluidic interaction for improved immuno-affinity based cell capture. The performance of the microdevice was evaluated by an image sequence analysis based in-house developed single cell tracking system. Individual cell tracking allowed in-depth analysis of the cell-chip surface interaction mechanism from hydrodynamic point of view. Simulation results were validated by using the hybrid device and the optimized surface functionalization procedure. Finally, the cell capture capability of this new generation microdevice was demonstrated by efficiently arresting cells from a HT29 cellline suspension.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Járvás, Gabor and Varga, Tamas. and Szigeti, Marton G. and Hajba, Laszlo and Fürjes, Peter and Rajta, István and Guttman, Andras (2017) Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture Part 2: Image sequence analysis based evaluation and biological application. ELECTROPHORESIS. ISSN 0173-0835, ESSN: 1522-2683 (In Press)
Kapcsolat:
https://doi.org/10.1002/elps.201700268
doi:10.1002/elps.201700268