Differentiation of strains identified as Bifidobacterium animalis subsp. lactis
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Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Differentiation of strains identified as Bifidobacterium animalis subsp. lactis
Létrehozó:
Markiewicz, L.H.
Biedrzycka, E.
Wasilewska, E.
Bielecka, M.
Kiadó:
Akadémiai Kiadó
Dátum:
2009
info:eu-repo/date/embargoEnd/2029-09-30
Téma:
QD01 Analytical chemistry / analitikai kémia
Tartalmi leírás:
Six strains (Bi11, Bi30, Bi36, Bi50, Bi52 and Bi55) isolated from bio-yoghurts and two strains (KD10 and KD11) derived from human faeces were identified by genus- and species specific polymerase chain reaction (PCR) with reference to the type strains of
B. animalis
subsp.
lactis
DSM 10140 and
B. animalis
subsp.
animalis
DSM 20104. The isolates were differentiated by using
Bcu
I (
Spe
I),
Xba
I and
Dra
I endonucleases for subsequent pulsed field gel electrophoresis (PFGE) technique and by API 50 CHL tests.All the isolates tested were classified to
B. animalis
subsp. lactis species. The reliable identification as
B. animalis
subsp.
lactis
(by PCR with Bflact2/Bflact5 primers), however, required confirmation by a negative result of
B. animalis
subsp.
animalis
-specific PCR.Differentiation of the
B. animalis
subsp.
lactis
isolates with PFGE method enabled to distinguish KD11 strain with all the restriction enzymes applied, and Bi11 and Bi30 — exclusively with
Dra
I and
Spe
I enzymes, respectively. The biochemical tests, however, revealed that all the strains tested were characterised by a unique fermentation pattern. It was concluded that differentiation of the
B. animalis
subsp.
lactis
strains should be carried out on the basis of both genetic and phenotypic features.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Markiewicz, L.H. and Biedrzycka, E. and Wasilewska, E. and Bielecka, M. (2009) Differentiation of strains identified as Bifidobacterium animalis subsp. lactis. Acta Alimentaria, 38 (3). pp. 293-301. ISSN 0139-3006
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess