Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition
  • Metaadatok
Tartalom: http://real.mtak.hu/33053/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition
Létrehozó:
Jetzschmann, Katharina J.
JĂĄgerszki, Gyula
Dechtrirat, Decha
Yarman, A.
Gajovic-Eichelmann, Nenad
GyurcsĂĄnyi, Ervin RĂłbert
Kiadó:
Wiley-VCH Verlag
Dátum:
2015
Téma:
QD01 Analytical chemistry / analitikai kĂŠmia
QH3011 Biochemistry / biokĂŠmia
Tartalmi leírás:
Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from ≈0.04 × 10−6 to 0.4 × 10−6m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 × 10−7m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the “shape-specific” MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.
Nyelv:
angol
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
text
Azonosító:
Jetzschmann, Katharina J. and JĂĄgerszki, Gyula and Dechtrirat, Decha and Yarman, A. and Gajovic-Eichelmann, Nenad and GyurcsĂĄnyi, Ervin RĂłbert (2015) Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. ADVANCED FUNCTIONAL MATERIALS, 25 (32). pp. 5178-5183. ISSN 1616-301X
Kapcsolat:
MTMT:2935395; doi:10.1002/adfm.201501900