NDA@SZTAKI

Activation energy associated with the electromigration of oligosaccharides through viscosity modifier and polymeric additive containing background electrolytes

Kerékgyártó, Márta

Járvás, Gábor

Novák, Levente

Guttman, András

Wiley-VCH Verlag

2015

QD01 Analytical chemistry / analitikai kémia

QD02 Physical chemistry / fizikai kémia

The activation energy related to the electromigration of oligosaccharides can be determined from their measured electrophoretic mobilities at different temperatures. The effects of a viscosity modifier (ethylene glycol) and a polymeric additive (linear polyacrylamide) on the electrophoretic mobility of linear sugar oligomers with alpha1–4 linked glucose units (maltooligosaccharides) were studied in CE using the activation energy concept. The electrophoretic separations of 8-aminopyrene-1,3,6-trisulfonate-labeledmaltooligosaccharides were monitored by LIF detection in the temperature range of 20–50°C, using either 0–60% ethylene glycol (viscosity modifier) or 0–3% linear polyacrylamide (polymeric additive)containing BGEs. Activation energy curves were constructed based on the slopes of the Arrhenius plots. With the use of linear polyacrylamide additive, solute size-dependent activation energy variations were found for the maltooligosaccharides with polymerization degrees below and above maltoheptaose (DP 7), probably due to molecular conformation changes and possible matrix interaction effects.

magyar

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/32100/1/elps5707.pdf

Kerékgyártó, Márta and Járvás, Gábor and Novák, Levente and Guttman, András (2015) Activation energy associated with the electromigration of oligosaccharides through viscosity modifier and polymeric additive containing background electrolytes. ELECTROPHORESIS, 37 (4). pp. 573-578. ISSN 0173-0835, ESSN: 1522-2683

http://real.mtak.hu/32100/

http://dx.doi.org/10.1002/elps.201500394

doi:10.1002/elps.201500394