Is less more? Lessons from aptamer selection strategies
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Tartalom: http://www.sciencedirect.com/science/article/pii/S0731708...
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published




Type = Article
Cím:
Is less more? Lessons from aptamer selection strategies
Létrehozó:
Szeitner, Zsuzsanna
András, Judit
Gyurcsányi, Ervin Róbert
Mészáros, Tamás
Dátum:
2014-12
Téma:
Q1 Science (General) / természettudomány általában
QD Chemistry / kémia
QD01 Analytical chemistry / analitikai kémia
QR180 Immunology / immunológia
Tartalmi leírás:
Aptamers have many inherent advantages originating from their in vitro selection and tailored chemical synthesis that makes them appealing alternatives of antibodies in bioaffinity assays. However, what ultimately matters, and that is the prerequisite to give way to all these advantages, is how well, and how selectively the aptamers bind to their targets. With the aptamer selection largely in the hand of life scientists, analytical chemists focused mostly on methodological development of aptamer-based assays using a fairly restricted number of aptamers to prove their concepts. However, ideally the development of an aptamer-based assay should start from the selection of aptamers to ensure their proper functionality in real samples. For instance information on the sample matrix can be implemented within counter-selection steps to discard aptamer candidates that show cross-reactivity to matrix components or critical interferents. In general, a larger consideration of the analytical use during selection and characterization of aptamers have been shown to increase the applicability of aptamers. Therefore, this review is a short, subjective view on trends in aptamer development highlighting factors to consider during their selection for a successful analytical application.
Típus:
Article
PeerReviewed
Formátum:
text
Azonosító:
Szeitner, Zsuzsanna and András, Judit and Gyurcsányi, Ervin Róbert and Mészáros, Tamás (2014) Is less more? Lessons from aptamer selection strategies. Journal of Pharmaceutical and Biomedical Analysis, 101. pp. 58-65.
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