Polyethylene imine-based receptor immobilization for label free bioassays
  • Metaadatok
Tartalom: http://www.sciencedirect.com/science/article/pii/S0925400...
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published




Type = Article
Cím:
Polyethylene imine-based receptor immobilization for label free bioassays
Létrehozó:
Kurunczi, Sándor
Hainard, Alexandre
Juhász, Krisztina
Patkó, Dániel
Orgovan, Norbert
Truck, Natacha
Sanchez, Jean-Charles
Horváth, Róbert
Kiadó:
Elsevier
Dátum:
2013
Téma:
QD01 Analytical chemistry / analitikai kémia
R1 Medicine (General) / orvostudomány általában
TD Environmental technology. Sanitary engineering / környezetvédelem, hulladékkezelés, egészségügyi mérnöki technika (ivóvízellátási és szennyvízkezelési technika)
TP Chemical technology / vegyipar, vegyészeti technológia
Tartalmi leírás:
Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the
label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched
PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups
of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated
anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully
performed in the low ?g/ml concentrations using a label free optical waveguide biosensor.
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Kurunczi, Sándor and Hainard, Alexandre and Juhász, Krisztina and Patkó, Dániel and Orgovan, Norbert and Truck, Natacha and Sanchez, Jean-Charles and Horváth, Róbert (2013) Polyethylene imine-based receptor immobilization for label free bioassays. SENSORS AND ACTUATORS B-CHEMICAL, 181. pp. 71-76. ISSN 0925-4005
Kapcsolat:
FP7-ICT4-P3SENS (248304)
Létrehozó:
info:eu-repo/semantics/openAccess