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Bejelentkezés
Kapcsolat
Polyethylene imine-based receptor immobilization for label free bioassays |
Tartalom: | http://www.sciencedirect.com/science/article/pii/S0925400... |
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Archívum: | MTA Könyvtár |
Gyűjtemény: |
Status = Published
Type = Article |
Cím: |
Polyethylene imine-based receptor immobilization for label free bioassays
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Létrehozó: |
Kurunczi, Sándor
Hainard, Alexandre
Juhász, Krisztina
Patkó, Dániel
Orgovan, Norbert
Truck, Natacha
Sanchez, Jean-Charles
Horváth, Róbert
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Kiadó: |
Elsevier
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Dátum: |
2013
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Téma: |
QD01 Analytical chemistry / analitikai kémia
R1 Medicine (General) / orvostudomány általában
TD Environmental technology. Sanitary engineering / környezetvédelem, hulladékkezelés, egészségügyi mérnöki technika (ivóvízellátási és szennyvízkezelési technika)
TP Chemical technology / vegyipar, vegyészeti technológia
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Tartalmi leírás: |
Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the
label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low ?g/ml concentrations using a label free optical waveguide biosensor. |
Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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Formátum: |
text
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Azonosító: |
Kurunczi, Sándor and Hainard, Alexandre and Juhász, Krisztina and Patkó, Dániel and Orgovan, Norbert and Truck, Natacha and Sanchez, Jean-Charles and Horváth, Róbert (2013) Polyethylene imine-based receptor immobilization for label free bioassays. SENSORS AND ACTUATORS B-CHEMICAL, 181. pp. 71-76. ISSN 0925-4005
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Kapcsolat: |
FP7-ICT4-P3SENS (248304)
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Létrehozó: |
info:eu-repo/semantics/openAccess
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