A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS
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Tartalom: http://real.mtak.hu/75166/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS
Létrehozó:
Varga, Andrea
Bata, ZsĂłfia
Csuka, Pál
Bordea, Diana Monica
G. Vértessy, Beáta
Irimie, Florin Dan
Poppe, László
Bencze, László Csaba
Kiadó:
Babes-Bolyai University
Dátum:
2017
Téma:
QD04 Organic chemistry / szerves kémia
Tartalmi leírás:
This study describes cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E.coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Varga, Andrea and Bata, Zsófia and Csuka, Pál and Bordea, Diana Monica and G. Vértessy, Beáta and Irimie, Florin Dan and Poppe, László and Bencze, László Csaba (2017) A NOVEL PHENYLALANINE AMMONIA-LYASE FROM KANGIELLA KOREENSIS. STUDIA UNIVERSITATIS BABES-BOLYAI SERIES CHEMIA, 62 (3). pp. 293-308. ISSN 0039-3401
Kapcsolat:
https://doi.org/10.24193/subbchem.2017.3.25
MTMT:3217504; doi:10.24193/subbchem.2017.3.25