A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions. (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions.

Metaadatok

Tartalom:	http://real.mtak.hu/74879/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions.
Létrehozó:	SzabÃ³, Edit TÃ¼rk, DÃ³ra Telbisz, Ãgnes Kucsma, NÃ³ra HorvÃ¡th, TamÃ¡s SzakÃ¡cs, Gergely Homolya, LÃ¡szlÃ³ Sarkadi, BalÃ¡zs VÃ¡rady, GyÃ¶rgy
Kiadó:	Public Library of Science (PLoS)
Dátum:	2018
Téma:	R850-854 Experimental medicine / kisÃ©rleti orvostudomÃ¡ny
Tartalmi leírás:	ABC multidrug transporters are key players in cancer multidrug resistance and in general xenobiotic elimination, thus their functional assays provide important tools for research and diagnostic applications. In this study we have examined the potential interactions of three key human ABC multidrug transporters with PhenGreen diacetate (PGD), a cell permeable fluorescent metal ion indicator. The non-fluorescent, hydrophobic PGD rapidly enters the cells and, after cleavage by cellular esterases, in the absence of quenching metal ions, PhenGreen (PG) becomes highly fluorescent. We found that in cells expressing functional ABCG2, ABCB1, or ABCC1 transporters, cellular PG fluorescence is strongly reduced. This fluorescence signal in the presence of specific transporter inhibitors is increased to the fluorescence levels in the control cells. Thus the PG accumulation assay is a new, unique tool for the parallel determination of the function of the ABCG2, ABCB1, and ABCC1 multidrug transporters. Since PG has very low cellular toxicity, the PG accumulation assay also allows the selection, separation and culturing of selected cell populations expressing either of these transporters.
Nyelv:	angol
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/74879/1/journal.pone.0190629.pdf SzabÃ³, Edit and TÃ¼rk, DÃ³ra and Telbisz, Ãgnes and Kucsma, NÃ³ra and HorvÃ¡th, TamÃ¡s and SzakÃ¡cs, Gergely and Homolya, LÃ¡szlÃ³ and Sarkadi, BalÃ¡zs and VÃ¡rady, GyÃ¶rgy (2018) A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions. PLOS ONE, 13 (1). pp. 1-15. ISSN 1932-6203
Kapcsolat:	http://real.mtak.hu/74879/ https://doi.org/10.1371/journal.pone.0190629 MTMT:3319075; doi:10.1371/journal.pone.0190629