Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics

Metaadatok

Tartalom:	http://real.mtak.hu/73308/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics
Létrehozó:	TÃ³th, Eszter A. Oszvald, ÃdÃ¡m PÃ©ter, MÃ¡ria Balogh, GÃ¡bor Osteikoetxea-MolnÃ¡r, AnikÃ³ BozÃ³, TamÃ¡s SzabÃ³-Meleg, Edina Nyitrai, MiklÃ³s Vigh, LÃ¡szlÃ³ MatkÃ³, JÃ¡nos
Dátum:	2017
Téma:	QH3011 Biochemistry / biokÃ©mia QH3020 Biophysics / biofizika
Tartalmi leírás:	Nanotubes (NTs) are thin, long membranous structures forming novel, yet poorly known communication pathways between various cell types. Key mechanisms controlling their growth still remained poorly understood. Since NT-forming capacity of immature and mature B cells was found largely different, we investigated how lipid composition and molecular order of the membrane affect NT-formation. Screening B cell lines with various differentiation stages revealed that NT-growth linearly correlates with membrane ganglioside levels, while it shows maximum as a function of cholesterol level. NT-growth of B lymphocytes is promoted by raftophilic phosphatidylcholine and sphingomyelin species, various glycosphingolipids, and docosahexaenoic acid-containing inner leaflet lipids, through supporting membrane curvature, as demonstrated by comparative lipidomic analysis of mature versus immature B cell membranes. Targeted modification of membrane cholesterol and sphingolipid levels altered NT-forming capacity confirming these findings, and also highlighted that the actual lipid raft number may control NT-growth via defining the number of membrane-F-actin coupling sites. Atomic force microscopic mechano-manipulation experiments further proved that mechanical properties (elasticity or bending stiffness) of B cell NTs also depend on the actual membrane lipid composition. Data presented here highlight importance of the lipid side in controlling intercellular, nanotubular, regulatory communications in the immune system.
Nyelv:	angol
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/73308/1/TothBBAMolCellBiolLipmanu.pdf TÃ³th, Eszter A. and Oszvald, ÃdÃ¡m and PÃ©ter, MÃ¡ria and Balogh, GÃ¡bor and Osteikoetxea-MolnÃ¡r, AnikÃ³ and BozÃ³, TamÃ¡s and SzabÃ³-Meleg, Edina and Nyitrai, MiklÃ³s and Vigh, LÃ¡szlÃ³ and MatkÃ³, JÃ¡nos (2017) Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1862 (9). pp. 991-1000. ISSN 1388-1981
Kapcsolat:	http://real.mtak.hu/73308/ https://doi.org/10.1016/j.bbalip.2017.06.011 MTMT:3243164; doi:10.1016/j.bbalip.2017.06.011