| Tartalom: |
http://real.mtak.hu/71811/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats
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| Létrehozó: |
Fábián, Renáta
Kovács, András
Stéger, Viktor
Frank, Krisztián
Egerszegi, István
Oláh, János
Bodó, Szilárd
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| Kiadó: |
Akadémiai Kiadó
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| Dátum: |
2017
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| Téma: |
SV Veterinary science / állatorvostudomány
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| Tartalmi leírás: |
The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
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| Nyelv: |
magyar
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| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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| Formátum: |
text
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| Azonosító: |
Fábián, Renáta and Kovács, András and Stéger, Viktor and Frank, Krisztián and Egerszegi, István and Oláh, János and Bodó, Szilárd (2017) X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats. Acta Veterinaria Hungarica, 65 (4). pp. 500-504. ISSN 0236-6290
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| Kapcsolat: |
https://doi.org/10.1556/004.2017.047
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