Direct mapping of melanoma cell - endothelial cell interactions
  • Metaadatok
Tartalom: http://real.mtak.hu/71032/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
Direct mapping of melanoma cell - endothelial cell interactions
Létrehozó:
Varga, BĂ©la
Fazakas, Csilla
Molnár, Judit
Wilhelm, Imola Mária
Domokos, RĂ©ka A.
Krizbai, István Adorján
Szegletes, Zsolt
Váró, György
VĂ©gh, Attila Gergely
Kiadó:
Wiley
Dátum:
2017
Téma:
QH3015 Molecular biology / molekuláris biológia
RC0254 Neoplasms. Tumors. Oncology (including Cancer) / daganatok, tumorok, onkolĂłgia
RL Dermatology / bőrgyógyászat
Tartalmi leírás:
The most life-threatening aspect of cancer is metastasis; cancer patient mortality is mainly due to metastasis. Among all metastases, presence of brain metastasis is one with the poorest prognosis; the median survival time can be counted in months. Therefore, prevention or decreasing their incidence would be highly desired both by patients and physicians. Metastatic cells invading the brain must breach the cerebral vasculature, primarily the blood-brain barrier. The key step in this process is the establishment of firm adhesion between the cancer cell and the cerebral endothelial layer. Using the atomic force microscope, a high-resolution force spectrograph, our aim was to explore the connections among the cell morphology, cellular mechanics, and biological function in the process of transendothelial migration of metastatic cancer cells. By immobilization of a melanoma cell to an atomic force microscope's cantilever, intercellular adhesion was directly measured at quasi-physiological conditions. Hereby, we present our latest results by using this melanoma-decorated probe. Binding characteristics to a confluent layer of brain endothelial cells was directly measured by means of single-cell force spectroscopy. Adhesion dynamics and strength were characterized, and we present data about spatial distribution of elasticity and detachment strength. These results highlight the importance of cellular mechanics in brain metastasis formation and emphasize the enormous potential toward exploration of intercellular dynamic-related processes.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Varga, Béla and Fazakas, Csilla and Molnár, Judit and Wilhelm, Imola Mária and Domokos, Réka A. and Krizbai, István Adorján and Szegletes, Zsolt and Váró, György and Végh, Attila Gergely (2017) Direct mapping of melanoma cell - endothelial cell interactions. JOURNAL OF MOLECULAR RECOGNITION, 30 (6). pp. 1-6. ISSN 0952-3499
Kapcsolat:
https://doi.org/10.1002/jmr.2603
MTMT:3167394; doi:10.1002/jmr.2603