| Tartalom: |
http://real.mtak.hu/70200/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
A novel poly(3-hydroxybutyrate)-degrading
Streptoverticillium kashmirense
AF1 isolated from soil and purification of PHB-depolymerase
|
| Létrehozó: |
Shah, A.
Hasan, Fariha
Hameed, A.
Ahmed, Safia
|
| Kiadó: |
Akadémiai Kiadó
|
| Dátum: |
2008
info:eu-repo/date/embargoEnd/2028-12-31
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| Téma: |
QH Natural history / természetrajz
|
| Tartalmi leírás: |
A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This
organism, identified as
Streptoverticillium kashmirense
AF1, secreted PHB depolymerases both on solid
as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The
optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 °C, 1% substrate concen-
tration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase
was purified by gel permeation chromatography using Sephadex G-75. The
Streptoverticillium kash-
mirense
AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45
kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO
2
evolved in the test
and control calculated gravimetrically through Sturm test indicated the degradative capabilities of
Streptoverticillium kashmirense
AF1.
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| Nyelv: |
magyar
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| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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| Formátum: |
text
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| Azonosító: |
Shah, A. and Hasan, Fariha and Hameed, A. and Ahmed, Safia (2008) A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase. Acta Biologica Hungarica, 59 (4). pp. 489-499. ISSN 0236-5383
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| Kapcsolat: |
https://doi.org/10.1556/ABiol.59.2008.4.9
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| Létrehozó: |
info:eu-repo/semantics/embargoedAccess
|
