NDA@SZTAKI

Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum

PĂłcsi, I.

Molnár, Zs.

Pusztahelyi, T.

Varecza, Z.

Emri, T.

Akadémiai Kiadó

2007

info:eu-repo/date/embargoEnd/2027-12-31

QH Natural history / természetrajz

The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high β-lactam producer
Penicillium chrysogenum
NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbonstarving cultures of the low-penicillin-producer strain
P. chrysogenum
ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type
P. chrysogenum
cultures. The mycelium→yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, which was a consequence of industrial strain development. Paradoxically, the development of high β-lactam productivity resulted in a high intracellular GSH level and, concomitantly, in an increased γ-glutamyltranspeptidase (i.e. GSH-decomposing) activity in the autolytic phase of growth of
P. chrysogenum
NCAIM 00237. The hypothesized causal connection between GSH metabolism and cell morphology, if verified, may help us in future metabolic engineering of industrially important filamentous fungi.

magyar

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/70136/1/abiol.58.2007.4.10.pdf

Pócsi, I. and Molnár, Zs. and Pusztahelyi, T. and Varecza, Z. and Emri, T. (2007) Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum. Acta Biologica Hungarica, 58 (4). pp. 431-440. ISSN 0236-5383

http://real.mtak.hu/70136/

https://doi.org/10.1556/ABiol.58.2007.4.10

info:eu-repo/semantics/embargoedAccess