A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination
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Tartalom: http://real.mtak.hu/69842/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination
Létrehozó:
Kelemen, K.
PĂ©czely, P.
Szőke, Zs.
Ladjánszky, V.
Kiadó:
Akadémiai Kiadó
Dátum:
2003
info:eu-repo/date/embargoEnd/2023-12-31
Téma:
QH Natural history / természetrajz
Tartalmi leírás:
In a comparative study, a relatively simple and high sensitivity method was developed for analysis of testosterone-equivalent(s) in the faeces of different bird species. To determine the recovery of extractions and purifications, tritium-labelled testosterone was added to the wet samples. Then the samples were treated with sodium dodecil sulphate (SDS), an emulsificator to “open-up” the complex, lipid-coated particles of faecal samples. This emulsification resulted in the decrease of the quantity of interfering substances after diethyl-ether extraction and the linearity of the measured testosterone equivalents from aliquots in the range of 2 and 10 mg of faeces. In the RIA, we applied a group specific polyclonal testosterone antibody which cross-reacted with reduced metabolites and at a certain level with sulphate conjugates as well. The use of Helix enzymes did not modified significantly the results of the analysis relating to a low level of conjugated androgens in the faecal extracts. The biological validity of the method was tested on domestic cockerels, where between the plasma and faecal testosterone values a four hours phase shift was observed, with a correlation of 0.6355. This method is suitable for “non invasive”, behavioural-ethological studies.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Kelemen, K. and Péczely, P. and Szőke, Zs. and Ladjánszky, V. (2003) A comparative methodical study of the faecal steroid analysis on birds: looking for a valid method of testosterone determination. Acta Biologica Hungarica, 54 (3-4). pp. 285-298. ISSN 0236-5383
Kapcsolat:
https://doi.org/10.1556/ABiol.54.2003.3-4.7
Létrehozó:
info:eu-repo/semantics/embargoedAccess