Triggering of a plant molecular defense mechanism: Increase in gene expression levels of transgene gsh I and poplar gene gsh 1 ( Populus × canescens ) by response to the DNA demethylating drug DHAC — an qRT-PCR analysis
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Type = Article
Cím:
Triggering of a plant molecular defense mechanism: Increase in gene expression levels of transgene gsh I and poplar gene gsh 1 ( Populus × canescens ) by response to the DNA demethylating drug DHAC — an qRT-PCR analysis
Létrehozó:
Bittsánszky, A.
Gyulai, G.
Malone, R. P.
Gullner, G.
Kiss, J.
Czakó, M.
Márton, L.
Heszky, L.
Kőmíves, T.
Kiadó:
Akadémiai Kiadó
Dátum:
2007
info:eu-repo/date/embargoEnd/2027-12-31
Téma:
QH540 Ecology / ökológia
QK10 Plant physiology / növényélettan
QL01 Systematic zoology / állatrendszertan
Tartalmi leírás:
As DNA methylation patterns are inherited (‘epigenetic memory’) gshI transgenic poplar (Populus×canescens) clones (11
ggs
and 6
Lgl
) were treated with the DNA demethylating drug DHAC (5,6-dihydro-5′-azacytidine hydrochloride) at 10−4
M for 7 days in order to study acquired plant molecular defense mechanisms in novel plant sources. In this study, the response of relative gene expression levels of transgene
gsh
I and poplar gene
gsh
1 to DHAC treatment were analyzed by qRT-PCR (
q
uantitative
r
everse
t
ranscriptase PCR). High expression levels of transgene
gsh
I were observed in the 6
Lgl
clone (13.5-fold increase) compared to 11
ggs
(1.0) sample. The expression level doubled (1.8-fold increase) in the DHAC-treated 6
Lgl
samples but not in the 11
ggs
clone (0.4-fold). Contrary to this, the relative copy number of transgene
gsh
I in the 6
Lgl
clone was found to be 60% less (1.0) than in the 11
ggs
sample (1.6). Relative expression level of endogenous poplar gene
gsh
1 showed significantly higher responsiveness to DHAC-induced demethylation than the transgene
gsh
I with the highest expression level in the untransformed WT poplar (19.7-fold increase) compared to transformed clones of 6
Lgl
(8.7-fold increase) and 11
ggs
(2.5-fold increase), respectively. Competition in the reactivation capacity between transgene
gsh
I and poplar
gsh
1 of 6
Lgl
clone was also observed as the relative gene expression level of transgene
gsh
I increased from a high relative expression level (13.5) up by about twofold (1.8 times) rate (to 23.7) compared to poplar
gsh
1 gene that increased by an 8.7 increment from a lower level (1.6 rel. expression) to 13.9.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Bittsánszky, A. and Gyulai, G. and Malone, R. P. and Gullner, G. and Kiss, J. and Czakó, M. and Márton, L. and Heszky, L. and Kőmíves, T. (2007) Triggering of a plant molecular defense mechanism: Increase in gene expression levels of transgene gsh I and poplar gene gsh 1 ( Populus × canescens ) by response to the DNA demethylating drug DHAC — an qRT-PCR analysis. Acta Phytopathologica et Entomologica Hungarica, 42 (2). pp. 235-243. ISSN 0238-1249
Kapcsolat:
https://doi.org/10.1556/APhyt.42.2007.2.6
Létrehozó:
info:eu-repo/semantics/embargoedAccess