| Tartalom: |
http://real.mtak.hu/64673/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Beyond homopolymer errors: a systematic investigation of Nanopore-based DNA sequencing characteristics using HLA-DQA2
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| Létrehozó: |
Sárközy, Péter
Molnár, Viktor
Fogl, DĂłra
Szalai, Csaba
Antal, PĂ©ter
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| Dátum: |
2017
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| Téma: |
QA75 Electronic computers. Computer science / számĂtástechnika, számĂtĂłgĂ©ptudomány
QA76 Computer software / programozás
TK Electrical engineering. Electronics Nuclear engineering / elektrotechnika, elektronika, atomtechnika
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| Tartalmi leírás: |
Electronic, nanopore based single molecule real-time DNA
sequencing technology offers very long, albeit lower accuracy
reads in sharp contrast to existing next-generation sequencing
methods, which offer short, high-accuracy reads in abundance.
We provide a systematic review of the error characteristics of
this new sequencing platform, and demonstrate the most challenging
aspects in the field of whole gene sequencing through
the human HLA-DQA2 gene using long-range PCR products
on multiplexed samples. We consider the limitations of these
errors for the applications of this technology, and also indicate
prospective improvements and expected thresholds with respect
to these errors.
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| Nyelv: |
angol
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| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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| Formátum: |
text
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| Azonosító: |
Sárközy, Péter and Molnár, Viktor and Fogl, Dóra and Szalai, Csaba and Antal, Péter (2017) Beyond homopolymer errors: a systematic investigation of Nanopore-based DNA sequencing characteristics using HLA-DQA2. Periodica Polytechnica Electrical Engineering and Computer Science, 61 (3). pp. 231-237. ISSN 20645260
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