Ugrás a tartalomhoz

 

Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding

  • Metaadatok
Tartalom: http://real.mtak.hu/63409/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding
Létrehozó:
Ecsédi, Péter
Kiss, Bence
Gógl, Gergő
Radnai, László
Buday, László
Koprivanecz, Kitti
Liliom, Károly
Leveles, Ibolya
Vértessy G., Beáta
Jeszenői, Norbert
Hetényi, Csaba
Schlosser, Gitta (Vácziné)
Katona, Gergely
Nyitray, László
Kiadó:
Elsevier
Dátum:
2017-08-01
Téma:
QD05 Crystallography / kristálytan
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
Tartalmi leírás:
Annexin A2 (ANXA2) has a versatile role in membrane-associated functions including membrane aggregation, endo- and exocytosis, and it is regulated by post-translational modifications and protein-protein interactions through the unstructured N-terminal domain (NTD). Our sequence analysis revealed a short motif responsible for clamping the NTD to the C-terminal core domain (CTD). Structural studies indicated that the flexibility of the NTD and CTD are interrelated and oppositely regulated by Tyr24 phosphorylation and Ser26Glu phosphomimicking mutation. The crystal structure of the ANXA2-S100A4 complex showed that asymmetric binding of S100A4 induces dislocation of the NTD from the CTD and, similar to the Ser26Glu mutation, unmasks the concave side of ANXA2. In contrast, pTyr24 anchors the NTD to the CTD and hampers the membrane-bridging function. This inhibition can be restored by S100A4 and S100A10 binding. Based on our results we provide a structural model for regulation of ANXA2-mediated membrane aggregation by NTD phosphorylation and S100 binding.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Ecsédi, Péter and Kiss, Bence and Gógl, Gergő and Radnai, László and Buday, László and Koprivanecz, Kitti and Liliom, Károly and Leveles, Ibolya and Vértessy G., Beáta and Jeszenői, Norbert and Hetényi, Csaba and Schlosser, Gitta (Vácziné) and Katona, Gergely and Nyitray, László (2017) Regulation of the Equilibrium between Closed and Open Conformations of Annexin A2 by N-Terminal Phosphorylation and S100A4-Binding. STRUCTURE, 25. p. 1. ISSN 0969-2126
Kapcsolat:
https://doi.org/10.1016/j.str.2017.06.001
doi: 10.1016/j.str.2017.06.001