Characterization of Staphylococcus aureus strains isolated from raw milk of bovine subclinical mastitis in Tehran and Mashhad
  • Metaadatok
Tartalom: http://real.mtak.hu/62891/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Characterization of Staphylococcus aureus strains isolated from raw milk of bovine subclinical mastitis in Tehran and Mashhad
Létrehozó:
Sahebekhtiari, Navid
Nochi, Zahra
Eslampour, Mohammad Amin
Dabiri, Hossein
Bolfion, Mehdi
Taherikalani, Morovat
Khoramian, Babak
Zali, Mohammad Reza
Emaneini, Mohammad
Kiadó:
Akadémiai Kiadó
Dátum:
2011
info:eu-repo/date/embargoEnd/2031-06-30
Téma:
QR Microbiology / mikrobiolĂłgia
Tartalmi leírás:
Staphylococcus aureus is considered one of the most important food borne pathogens.A total of 111 isolates of S. aureus were cultured from raw milk samples during January 2009 to June 2009 from Tehran and Mashhad. The coagulase gene polymorphism and the prevalence of classical enterotoxin genes of S. aureus strains were determined by PCR-RFLP (restriction fragment length polymorphism) and Multiplex-PCR. Disk diffusion method was used to determine the susceptibility of isolates to antimicrobial agents as instructed by Clinical and Laboratory Standards Institute.Sixty-seven % of the isolates harboured one or more enterotoxin genes. The most prevalent gene was sec, found in 59 % of the isolates. Approximately 8% of the isolates were positive for sea, seb and sed genes. Only one isolate had see gene. The rate of coexistence of enterotoxin genes was 14%. All S. aureus isolates were susceptible to ciprofloxacin, gentamicin, imipenem, minocycline, oxacillin and vancomycin. They were resistant to ampicillin (64%), penicillin (56%), clindamycin (22%), tetracycline (22%), doxycycline (19%), teicoplanin (13%), rifampin (2%) and trimethoprim-sulfamethoxazole (2%). On the basis of coagulase gene analysis of 111 S. aureus isolates, the PCR products of 56 isolates were digested with Alu I that produced three distinct patterns.These data indicate the high prevalence of enterotoxigenic S. aureus in raw bovine milk in Tehran and Mashhad, and highlight the importance of proper quality control of dairy products for public health.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Sahebekhtiari, Navid and Nochi, Zahra and Eslampour, Mohammad Amin and Dabiri, Hossein and Bolfion, Mehdi and Taherikalani, Morovat and Khoramian, Babak and Zali, Mohammad Reza and Emaneini, Mohammad (2011) Characterization of Staphylococcus aureus strains isolated from raw milk of bovine subclinical mastitis in Tehran and Mashhad. Acta Microbiologica et Immunologica Hungarica, 58 (2). pp. 113-121. ISSN 1217-8950
Kapcsolat:
https://doi.org/10.1556/AMicr.58.2011.2.4
Létrehozó:
info:eu-repo/semantics/embargoedAccess