| Tartalom: |
http://real.mtak.hu/62449/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
|
| Cím: |
An origin of transfer from
Lactococcus lactis
subsp.
lactis
causes rearrangement in the temperature sensitive plasmids pUCB3522 and pJRS290
|
| Létrehozó: |
Amani, M. D. Elahwany
|
| Kiadó: |
Akadémiai Kiadó
|
| Dátum: |
2007
info:eu-repo/date/embargoEnd/2027-12-31
|
| Téma: |
QR Microbiology / mikrobiolĂłgia
|
| Tartalmi leírás: |
Cloning of the
oriT
region derived from
Lactococcus lactis
subsp.
lactis
into plasmids is expected to convert them into mobilizable plasmids in a suitable genetic background. In some cases, however, the cloning of
oriT
causes deleterious DNA rearrangements. In the work presented,
oriT
was cloned into two temperature sensitive plasmids, pJRS290 and pUCB3522. The plasmids were transformed into
E. coli
and electroporated into
L. lactis
. Restriction digestion of the plasmids derived from the cloned transformed cells showed fragment sizes different from those expected, indicating that rearrangements had occurred. Furthermore, the inability to successfully transform
E. coli
with one of the plasmids supports this conclusion.
|
| Nyelv: |
magyar
|
| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
|
| Formátum: |
text
|
| Azonosító: |
Amani, M. D. Elahwany (2007) An origin of transfer from Lactococcus lactis subsp. lactis causes rearrangement in the temperature sensitive plasmids pUCB3522 and pJRS290. Acta Microbiologica et Immunologica Hungarica, 54 (4). pp. 413-420. ISSN 1217-8950
|
| Kapcsolat: |
https://doi.org/10.1556/AMicr.54.2007.4.7
|
| Létrehozó: |
info:eu-repo/semantics/embargoedAccess
|
