Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA

Metaadatok

Tartalom:	http://real.mtak.hu/62389/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA
Létrehozó:	NÃ©meth, Andrea Orgovan, Norbert SÃ³dar, Barbara W Osteikoetxea, Xabier PÃ¡lÃ³czi, Krisztina SzabÃ³-Taylor, Katalin Ã‰. Vukman, Krisztina V. Kittel, Ãgnes TuriÃ¡k, Lilla Wiener, ZoltÃ¡n TÃ³th, SÃ¡ra Drahos, LÃ¡szlÃ³ VÃ©key, KÃ¡roly Horvath, Robert BuzÃ¡s, Edit I.
Kiadó:	Nature Publishing Group
Dátum:	2017-08-15
Téma:	R850-854 Experimental medicine / kisÃ©rleti orvostudomÃ¡ny
Tartalmi leírás:	Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.
Nyelv:	magyar
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/62389/1/1_s41598-017-08392-1.pdf NÃ©meth, Andrea and Orgovan, Norbert and SÃ³dar, Barbara W and Osteikoetxea, Xabier and PÃ¡lÃ³czi, Krisztina and SzabÃ³-Taylor, Katalin Ã‰. and Vukman, Krisztina V. and Kittel, Ãgnes and TuriÃ¡k, Lilla and Wiener, ZoltÃ¡n and TÃ³th, SÃ¡ra and Drahos, LÃ¡szlÃ³ and VÃ©key, KÃ¡roly and Horvath, Robert and BuzÃ¡s, Edit I. (2017) Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. SCIENTIFIC REPORTS, 7 (1). pp. 1-16. ISSN 2045-2322
Kapcsolat:	http://real.mtak.hu/62389/ http://doi.org/10.1038/s41598-017-08392-1 doi:10.1038/s41598-017-08392-1