NDA@SZTAKI

Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultastructure

Geleta, Anna

KristĂłf, Z.

Maráz, Anna

Akadémiai Kiadó

2007

info:eu-repo/date/embargoEnd/2027-03-31

QR Microbiology / mikrobiolĂłgia

Scanning and transmission electron microscopic studies revealed the presence of slime-like, amorphous material on the surface of
Schizosaccahromyces pombe
RIVE 4-2-1 cells, independently, whether they were in flocculated or in non-flocculated state. Close contact of the adjacent cells via the merging outermost cell wall layers was found, however, only in the case of floc formation, which was induced by cultivating the cells in the presence of 6% (v/v) ethanol. Irreversible loss of the flocculation ability of the cells by treatment with proteinases suggests that proteinaceous cell surface molecules as lectins contribute to the cell-to-cell interaction during flocculation. Both proteinase K and pronase treatments removed a distinct outer layer of the cell wall, which indicated that the protein moieties of the phosphogalactomannan outer surface layer has a crucial role in the maintenance of cell wall integrity. In the case of lysing enzyme treatment the removal of the outermost layer was also observed as the first step of the cell wall digestion, while driselase treatment resulted in almost complete digestion of the cell wall.

magyar

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/62330/1/amicr.54.2007.1.4.pdf

Geleta, Anna and Kristóf, Z. and Maráz, Anna (2007) Cell wall ultrastructure of flocculent and non-flocculent Schizosaccharomyces pombe strains. Effect of cell wall hydrolysing enzymes on flocculation and cell wall ultastructure. Acta Microbiologica et Immunologica Hungarica, 54 (1). pp. 35-46. ISSN 1217-8950

http://real.mtak.hu/62330/

https://doi.org/10.1556/AMicr.54.2007.1.4

info:eu-repo/semantics/embargoedAccess