| Tartalom: |
http://real.mtak.hu/62251/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Cloning, expression and purification OF SmpB from Mycobacterium tuberculosis
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| Létrehozó: |
Kovács, L.
Megyeri, Klára
Juhász, Anna
Zaja, AnikĂł
Miczák, A.
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| Kiadó: |
Akadémiai Kiadó
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| Dátum: |
2004
info:eu-repo/date/embargoEnd/2024-11-30
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| Téma: |
QR Microbiology / mikrobiolĂłgia
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| Tartalmi leírás: |
SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations.
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| Nyelv: |
magyar
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| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
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| Formátum: |
text
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| Azonosító: |
Kovács, L. and Megyeri, Klára and Juhász, Anna and Zaja, Anikó and Miczák, A. (2004) Cloning, expression and purification OF SmpB from Mycobacterium tuberculosis. Acta Microbiologica et Immunologica Hungarica, 51 (3). pp. 297-302. ISSN 1217-8950
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| Kapcsolat: |
https://doi.org/10.1556/AMicr.51.2004.3.7
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| Létrehozó: |
info:eu-repo/semantics/embargoedAccess
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