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Cloning and sequence analysis of Mucor Circinelloides glyceraldehyde-3-phosphate dehydrogenase gene

  • Metaadatok
Tartalom: http://real.mtak.hu/62168/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
Cloning and sequence analysis of Mucor Circinelloides glyceraldehyde-3-phosphate dehydrogenase gene
Létrehozó:
Ács, Klåra
Kasza, Zs.
LukĂĄcs, GyĂśngyi
Schwab, H.
VĂĄgvĂślgyi, Cs.
Kiadó:
AkadĂŠmiai KiadĂł
Dátum:
2002
info:eu-repo/date/embargoEnd/2022-05-31
Téma:
QR Microbiology / mikrobiolĂłgia
Tartalmi leírás:
A genomic library of Mucor circinelloides ATCC 1216b has been constructed in Lambda Fix II vector. The library has an average insert site of 10 kb and covers the genome 12 times. The M. circinelloides gene encoding glyceraldehyde-3-phosphate dehydrogenase (gpd) was isolated from this library by hybridization of the recombinant phage clones with a gpd-specific gene probe generated by PCR reaction. The complete nucleotide sequence encodes a putative polypeptide chain of 339 amino acids interrupted by 3 introns. The predicted amino acid sequence of this gene shows a high degree of sequence similarity to the GPD proteins from other filamentous fungi. The promoter region, containing a consensus TATA and CAAT box and a 298 nucleotid long termination region were also determined.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Ács, Klåra and Kasza, Zs. and Lukåcs, GyÜngyi and Schwab, H. and VågvÜlgyi, Cs. (2002) Cloning and sequence analysis of Mucor Circinelloides glyceraldehyde-3-phosphate dehydrogenase gene. Acta Microbiologica et Immunologica Hungarica, 49 (2-3). pp. 305-312. ISSN 1217-8950
Kapcsolat:
https://doi.org/10.1556/AMicr.49.2002.2-3.18
Létrehozó:
info:eu-repo/semantics/embargoedAccess