Zebrafish Androgen Receptor: Isolation, Molecular, and Biochemical Characterization
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Tartalom: http://real.mtak.hu/61383/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published




Type = Article
Cím:
Zebrafish Androgen Receptor: Isolation, Molecular, and Biochemical Characterization
Létrehozó:
Hossain, Mohammad Sorowar
Orbán, László
Kiadó:
Society for the Study of Reproduction
Dátum:
2008-02-01
Téma:
QH301 Biology / biolĂłgia
QH3015 Molecular biology / molekuláris biológia
QH426 Genetics / genetika, örökléstan
QL Zoology / állattan
Tartalmi leírás:
Androgens play an important role in male sexual differentiation and development. They exert their function by binding to and activating the androgen receptor (Ar), a member of the steroid hormone receptor superfamily. Here, we report on the isolation and characterization of zebrafish Ar. The complete transcript of zebrafish ar is 5.3 kb long encoding a putative polypeptide of 868 amino acids. Our experimental and bioinformatic analysis has found a single ar locus in zebrafish. Phylogenetic analysis using the ligand-binding domain showed that the zebrafish Ar clustered with its cyprinid orthologs to form a separate group, which was closer to the beta clade than to the alpha clade. Tissue-specific expression analysis revealed that the ar mRNA was expressed ubiquitously in all adult tissues tested, with sexually dimorphic expression in the gonad and muscle. While the ar transcript was maternally deposited into the embryo, signs of zygotic expression could be detected as early as 24 h after fertilization, and the expression level increased substantially afterwards. When analyzed during gonad development, the expression level of ar mRNA at 4 wk after fertilization was similar in both developing gonads but later became higher in the transforming testis, suggesting a potential role during male gonad differentiation. We also combined theoretical modeling with in vitro experiments to show that the zebrafish Ar is preferentially activated by 11-ketotestosterone.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Hossain, Mohammad Sorowar and Orbán, László (2008) Zebrafish Androgen Receptor: Isolation, Molecular, and Biochemical Characterization. Biology of Reproduction, 78 (2). pp. 361-369. ISSN 0006-3363, ESSN: 1529-7268
Kapcsolat:
https://doi.org/10.1095/biolreprod.107.062018