Colistin resistance associated with outer membrane protein change inÂ Klebsiella pneumoniae and Enterobacter asburiae (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Colistin resistance associated with outer membrane protein change inÂ Klebsiella pneumoniae and Enterobacter asburiae

Metaadatok

Tartalom:	http://real.mtak.hu/56035/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Colistin resistance associated with outer membrane protein change inÂ Klebsiella pneumoniae and Enterobacter asburiae
Létrehozó:	KÃ¡dÃ¡r, BÃ©la Kocsis, BÃ©la TÃ³th, Ãkos KristÃ³f, Katalin FelsÅ‘, PÃ©ter Kocsis, BÃ©la BÃ¶ddi, Katalin SzabÃ³, DÃ³ra
Kiadó:	AkadÃ©miai KiadÃ³
Dátum:	2017
Téma:	QR Microbiology / mikrobiolÃ³gia
Tartalmi leírás:	In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by two-dimensional gel electrophoresis and in-gel digestion. The MALDI-TOF/MS analysis of OMPs in the colistin-susceptible K. pneumoniae found 16Â kDa proteins belonging to the LysM domain/BON superfamily, as well as DNA starvation proteins, whereas OmpX and OmpW were detected in the colistin-resistant counterpart strains. OmpC and OmpW were detected in the colistin-susceptible E. asburiae, whereas OmpA and OmpX were identified in the colistin-resistant counterpart. This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae.
Nyelv:	magyar
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/56035/1/030.64.2017.017.pdf KÃ¡dÃ¡r, BÃ©la and Kocsis, BÃ©la and TÃ³th, Ãkos and KristÃ³f, Katalin and FelsÅ‘, PÃ©ter and Kocsis, BÃ©la and BÃ¶ddi, Katalin and SzabÃ³, DÃ³ra (2017) Colistin resistance associated with outer membrane protein change in Klebsiella pneumoniae and Enterobacter asburiae. Acta Microbiologica et Immunologica Hungarica, 64 (2). pp. 217-227. ISSN 1217-8950
Kapcsolat:	http://real.mtak.hu/56035/ https://doi.org/10.1556/030.64.2017.017