| Tartalom: |
http://real.mtak.hu/55851/ |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-cyclooctynylated Peptide Tags and as Fluorogenic Crosslinkers of Cyclooctynylated Proteins
|
| Létrehozó: |
Demeter, Orsolya
Kormos, Attila
Koehler, Christine
MezĹ, GĂĄbor
NĂŠmeth, Krisztina
Kele, PĂŠter
TakĂĄcs, Levente B.
Lemke, Edward A.
|
| Dátum: |
2017
|
| Téma: |
QD04 Organic chemistry / szerves kĂŠmia
QH301 Biology / biolĂłgia
|
| Tartalmi leírás: |
Herein we present the synthesis and
fl
uoro-
genic characterization of a series of double-quenched bisazide
cyanine probes with emission maxima between 565 and 580
nm that can participate in covalent, two-point binding
bioorthogonal tagging schemes in combination with bis-
cyclooctynylated peptides. Compared to other
fl
uorogenic
cyanines, these double-quenched systems showed remarkable
fl
uorescence intensity increase upon formation of cyclic dye
â
peptide conjugates. Furthermore, we also demonstrated that
these bisazides are useful
fl
uorogenic cross-linking platforms
that are able to form a covalent linkage between mono-
cyclooctynylated proteins.
|
| Nyelv: |
angol
|
| Típus: |
Article
PeerReviewed
info:eu-repo/semantics/article
|
| Formátum: |
text
|
| Azonosító: |
Demeter, Orsolya and Kormos, Attila and Koehler, Christine and MezĹ, GĂĄbor and NĂŠmeth, Krisztina and Kele, PĂŠter and TakĂĄcs, Levente B. and Lemke, Edward A. (2017) Bisazide Cyanine Dyes as Fluorogenic Probes for Bis-cyclooctynylated Peptide Tags and as Fluorogenic Crosslinkers of Cyclooctynylated Proteins. BIOCONJUGATE CHEMISTRY, in pre. in press. ISSN 1043-1802
|
| Kapcsolat: |
https://doi.org/10.1021/acs.bioconjchem.7b00178
MTMT:3219435; doi:10.1021/acs.bioconjchem.7b00178
|
