Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [3H]noradrenaline from a slice preparation (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [3H]noradrenaline from a slice preparation

Metaadatok

Tartalom:	http://real.mtak.hu/54825/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [3H]noradrenaline from a slice preparation
Létrehozó:	BorbÃ©ly, ZoltÃ¡n CsomÃ³, Benedek KrisztiÃ¡n Kittel, Ãgnes Gerber, GÃ¡bor Varga, GÃ¡bor Vizi, E. Szilveszter
Kiadó:	Elsevier
Dátum:	2017
Téma:	R850-854 Experimental medicine / kisÃ©rleti orvostudomÃ¡ny RS Pharmacy and materia medica / gyÃ³gyszerÃ©szet, gyÃ³gyÃ¡szati eszkÃ¶zÃ¶k
Tartalmi leírás:	We measured the ex vivo uptake and release of [3H]noradrenaline ([3H]NA) from perfused rat spinal cord slice preparations at 1, 3 and 14days after unilateral hemisection-induced spinal cord injury (SCI) compared with control slice preparations. After surgical hemisection under anaesthesia, the rats showed characteristic signs of hemiplegia, with no movement of the ipsilateral hindlimb. After 3days, the electron microscopy images showed overall degeneration of neuronal organelles and the myelin sheath, but the synapses seemed to be intact. In ex vivo experiments, the spinal cord injury did not influence uptake but increased [3H]NA release at rest and in response to axonal stimulation. The effect of a selective noradrenaline reuptake inhibitor, nisoxetine, was studied to identify the mechanisms underlying the increase in NA release. Nisoxetine potentiated stimulation-evoked [3H]NA release from the non-injured tissue, but it gradually lost its effectiveness after injury, depending on the time (1 and 3days) elapsed after hemisection, indicating that the noradrenaline transporter binding sites of the terminals become impaired after decentralisation.
Nyelv:	angol
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/54825/1/vizi.pdf BorbÃ©ly, ZoltÃ¡n and CsomÃ³, Benedek KrisztiÃ¡n and Kittel, Ãgnes and Gerber, GÃ¡bor and Varga, GÃ¡bor and Vizi, E. Szilveszter (2017) Effect of rat spinal cord injury (hemisection) on the ex vivo uptake and release of [3H]noradrenaline from a slice preparation. BRAIN RESEARCH BULLETIN, 131. pp. 150-155. ISSN 0361-9230
Kapcsolat:	http://real.mtak.hu/54825/ https://doi.org/10.1016/j.brainresbull.2017.04.007 MTMT:3239171; doi:10.1016/j.brainresbull.2017.04.007