Potentiometric sensing of nucleic acids using chemically modified nanopores
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Tartalom: http://real.mtak.hu/54550/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Potentiometric sensing of nucleic acids using chemically modified nanopores
Létrehozó:
Makra, István
Brajnovits, Alexandra
Jágerszki, Gyula
FĂĽrjes, PĂ©ter
Gyurcsányi, Ervin Róbert
Kiadó:
Royal Society of Chemistry
Dátum:
2017
Téma:
QC Physics / fizika
T2 Technology (General) / műszaki tudományok általában
Tartalmi leírás:
Unlike the overwhelming majority of nanopore sensors that are
based on the measurement of a transpore ionic current, here we
introduce a potentiometric sensing scheme and demonstrate its
application for the selective detection of nucleic acids. The
sensing concept uses the charge inversion that occurs in the
sensing zone of a nanopore upon binding of negatively charged
microRNA strands to positively charged peptide-nucleic acid
(PNA) modified nanopores. The initial anionic permselectivity
of PNA-modified nanopores is thus gradually changed to cationic
permselectivity, which can be detected simply by measuring the
nanoporous membrane potential. A quantitative theoretical
treatment of the potentiometric microRNA response is provided
based on the Nernst-Planck/Poisson model for the nanopore
system assuming first order kinetics for the nucleic acid
hybridization. An excellent correlation between the theoretical
and experimental results was observed, which revealed that the
binding process is focused at the nanopore entrance with
contributions from both in pore and out of pore sections of the
nanoporous membrane. The theoretical treatment is able to give
clear guidelines for further optimization of potentiometric
nanopore-based nucleic acid sensors by predicting the effect of
the most important experimental parameters on the potential
response.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Makra, István and Brajnovits, Alexandra and Jágerszki, Gyula and Fürjes, Péter and Gyurcsányi, Ervin Róbert (2017) Potentiometric sensing of nucleic acids using chemically modified nanopores. NANOSCALE, 9 (2). pp. 739-747. ISSN 2040-3364
Kapcsolat:
https://doi.org/10.1039/C6NR05886H
MTMT:3162240; doi:10.1039/C6NR05886H