NDA@SZTAKI

A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay

TÜlgyesi, Á.

Fekete, J.

Sharma, V.K.

Pálffi, É.

BĂŠkĂŠsi, K.

Lukonics, D.

Pleva, G.

AkadĂŠmiai KiadĂł

2014

QD01 Analytical chemistry / analitikai kĂŠmia

A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 μg kg^−1. All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 μg kg^−1 for CAP in each validated matrix, while the detection capability of the screening test was 0.15 μg kg^−1.

magyar

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/49850/1/aalim.43.2014.2.15.pdf

Tölgyesi, Á. and Fekete, J. and Sharma, V.K. and Pálffi, É. and Békési, K. and Lukonics, D. and Pleva, G. (2014) A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay. Acta Alimentaria, 43 (2). pp. 306-314. ISSN 0139-3006

http://real.mtak.hu/49850/

http://dx.doi.org/10.1556/AAlim.43.2014.2.15