A new, modern, cost-saving micro/macro method for the determination of serum fructosamine
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Tartalom: http://real.mtak.hu/49478/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
A new, modern, cost-saving micro/macro method for the determination of serum fructosamine
Létrehozó:
Oppel, KlĂĄra
BĂĄrdos, L.
Ferencz, A.
Lakner, Hajnalka
Simon, Judit
TemesvĂĄry, Kriszta
Karchesz, Krisztina
KulcsĂĄr, Margit
Kiadó:
AkadĂŠmiai KiadĂł
Dátum:
2000
Téma:
SV Veterinary science / ĂĄllatorvostudomĂĄny
Tartalmi leírás:
Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 µL samples and 200 µL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year. SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated ‘micro’ method as well as by the manual test tube ‘macro’ method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p < 0.001).
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Oppel, KlĂĄra and BĂĄrdos, L. and Ferencz, A. and Lakner, Hajnalka and Simon, Judit and TemesvĂĄry, Kriszta and Karchesz, Krisztina and KulcsĂĄr, Margit (2000) A new, modern, cost-saving micro/macro method for the determination of serum fructosamine. Acta Veterinaria Hungarica, 48 (3). pp. 285-291. ISSN 0236-6290
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