Ugrás a tartalomhoz

 

Differentiation of Mycoplasma gallisepticum strains using molecular methods

  • Metaadatok
Tartalom: http://real.mtak.hu/48784/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Differentiation of Mycoplasma gallisepticum strains using molecular methods
Létrehozó:
BĂ­rĂł, Judit
Erdei, Noémi
Székely, Ibolya
Stipkovits, L.
Kiadó:
Akadémiai Kiadó
Dátum:
2006
info:eu-repo/date/embargoEnd/2026-12-31
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Increasing use of Mycoplasma gallisepticum (MG) live vaccines has led to a need for the differentiation of MG strains. The MG strains MK-7, MS-16, S6, FS-9 and R strains and the MG live vaccine strain F were compared by random amplification of polymorphic DNA (RAPD) in this study. Using RAPD, different patterns were found among the MG strains. In addition to this, we examined the differentiating potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) primers targeted at the crmA, crmB, crmC, gapA, mgc2 and pvpA genes encoding cytadherence-related surface proteins. These proteins may take part in the pathogenesis of MG-induced disease. Differentiation of strain F is based on the identification of restriction enzyme sites in the PCR amplicons. Using HphI enzyme, crmC PCR amplicons produced different RFLP patterns. Digestion of amplicons of gapA-specific PCR with MboI enzyme also produced distinct patterns. Differences were observed among strains R and F by digestion of mgc2 PCR amplicons with HaeIII and VspI enzymes and digestion of pvpA PCR amplicons with AccI, PvuII and ScrFI endonucleases. This method can be used for the rapid differentiation of vaccine strain from wild strains. Differentiation of MG strains is a great advantage for diagnosticians or practitioners and it is useful for epidemiological studies.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Bíró, Judit and Erdei, Noémi and Székely, Ibolya and Stipkovits, L. (2006) Differentiation of Mycoplasma gallisepticum strains using molecular methods. Acta Veterinaria Hungarica, 54 (4). pp. 437-448. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess