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Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Varga, Erika

GajdĂłcsi, ErzsĂŠbet

Petz MakkosnĂŠ, Brigitta

Salamon, IldikĂł

Bali Papp, Ágnes

AkadĂŠmiai KiadĂł

2008

info:eu-repo/date/embargoEnd/2028-09-30

SV Veterinary science / ĂĄllatorvostudomĂĄny

The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent
in vitro
fertilisation; the resultant zygotes were then cultured
in vitro
for four days to assess embryonic development. In the first experiment,
in vitro
maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and
in vitro
fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

magyar

Article

PeerReviewed

info:eu-repo/semantics/article

text

http://real.mtak.hu/48431/1/avet.56.2008.3.13.pdf

Varga, Erika and Gajdócsi, ErzsÊbet and Petz MakkosnÊ, Brigitta and Salamon, Ildikó and Bali Papp, Ágnes (2008) Vitrification of in vitro matured oocytes of Mangalica and Large White pigs. Acta Veterinaria Hungarica, 56 (3). pp. 399-410. ISSN 0236-6290

http://real.mtak.hu/48431/

http://dx.doi.org/10.1556/AVet.56.2008.3.13

info:eu-repo/semantics/embargoedAccess