Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods
  • Metaadatok
Tartalom: http://real.mtak.hu/48402/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods
Létrehozó:
Jankovičová, Jana
Simon, Michal
Antalíková, Jana
Horovská, Ľubica
Kiadó:
Akadémiai Kiadó
Dátum:
2008
info:eu-repo/date/embargoEnd/2028-03-31
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst/FITC-
Pisum sativum
agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-PSA labelling (39.33%). The viability of sperm stained by the double staining method was 67.17%, and a higher portion of viable sperm (82.67%) was observed by staining with the Hoechst procedure (P < 0.01). On the basis of the results obtained it is concluded that both methods can be used for detecting the acrosome reaction of frozen-thawed bovine spermatozoa.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Jankovičová, Jana and Simon, Michal and Antalíková, Jana and Horovská, Ľubica (2008) Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods. Acta Veterinaria Hungarica, 56 (1). pp. 133-138. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess