Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification
  • Metaadatok
Tartalom: http://real.mtak.hu/48295/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification
Létrehozó:
Papis, Krzysztof
Korwin-Kossakowski, Maciej
Wenta-Muchalska, ElĹźbieta
Kiadó:
AkadĂŠmiai KiadĂł
Dátum:
2009
info:eu-repo/date/embargoEnd/2029-09-30
Téma:
SV Veterinary science / ĂĄllatorvostudomĂĄny
Tartalmi leírás:
In spite of their cryobiological efficacy, minimum-volume vitrification methods suffer from the risk of microbiological contamination and are technically and/or manually demanding. In this study, the effects of a traditional, slightly modified vitrification method and vitrification using supercooled liquid nitrogen (VitMaster) applied for rabbit morula-stage embryos were compared. Embryos were equilibrated in a solution containing 1,2-propanediol (2.72 M) and glycerol (1.36 M) for 7 min and vitrified in 0.25-ml insemination straws after 1-min exposure to a vitrification solution containing additionally 1.0 M sucrose. Cooling was performed in ‘normal’ or supercooled liquid nitrogen. Regardless of the cooling method applied, high
in vitro
survival and development rates of vitrified embryos were obtained. All embryos were intact after warming, and 61 out of 65 (93.8%) and 23 out of 24 (95.8%) embryos developed to the blastocyst stage after 48-h
in vitro
culture of embryos vitrified in ‘normal’ or supercooled liquid nitrogen, respectively. The results suggest higher developmental ability of embryos vitrified in supercooled liquid nitrogen (91.7%
vs
. 83.1% of embryos vitrified traditionally developed to more advanced, expanding and/or hatching blastocyst stages).
In vivo
survival rate, tested for the traditional vitrification system only, revealed that 36.8% of embryos developed to term. The results show promise for establishing a fully successful method for rabbit embryo vitrification.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Papis, Krzysztof and Korwin-Kossakowski, Maciej and Wenta-Muchalska, ElĹźbieta (2009) Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification. Acta Veterinaria Hungarica, 57 (3). pp. 411-416. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess