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A pulsed-field gel electrophoresis study of the genetic diversity of Campylobacter jejuni and Campylobacter coli in poultry flocks in Slovenia

  • Metaadatok
Tartalom: http://real.mtak.hu/48085/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
A pulsed-field gel electrophoresis study of the genetic diversity of Campylobacter jejuni and Campylobacter coli in poultry flocks in Slovenia
Létrehozó:
Gruntar, Igor
Ocepek, MatjaĹľ
Avberšek, Jana
Mićunović, Jasna
Pate, Mateja
Kiadó:
Akadémiai Kiadó
Dátum:
2010
info:eu-repo/date/embargoEnd/2030-03-31
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Campylobacter jejuni
and
C. coli
have recently become the most frequent cause of bacterial foodborne enteric infection in most industrialised countries. Consumption and handling of undercooked contaminated poultry meat was identified as an important risk factor for human campylobacteriosis. The aim of this study was to ascertain the genetic diversity of
C. jejuni
and
C. coli
strains isolated from poultry in Slovenia. A total of 68 isolates (42
C. jejuni
, 26
C. coli
) from faeces (n = 48), meat (n = 15) and skin/carcasses (n = 5) of chicken (n = 60) and turkey samples (n = 5) were analysed by pulsed-field gel electrophoresis.
Sma
I macrorestriction discriminated between
C. jejuni
and
C. coli
isolates.
C. jejuni
isolates exhibited a higher degree of diversity compared to
C. coli
isolates. In the
C. jejuni
group, a number of small clusters were apparent, while
C. coli
strains formed less but larger clusters. Additional
Kpn
I digestion of selected isolates resulted in poor subtyping. Strains with identical or very similar profiles were found on different farms, either in the same or different regions and time periods. Some of the clones indicated possible cross-contamination at slaughterhouses.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Gruntar, Igor and Ocepek, Matjaž and Avberšek, Jana and Mićunović, Jasna and Pate, Mateja (2010) A pulsed-field gel electrophoresis study of the genetic diversity of Campylobacter jejuni and Campylobacter coli in poultry flocks in Slovenia. Acta Veterinaria Hungarica, 58 (1). pp. 19-28. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess