Vitrification of early avian blastodermal cells with a new type of cryocontainer
  • Metaadatok
Tartalom: http://real.mtak.hu/47765/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Vitrification of early avian blastodermal cells with a new type of cryocontainer
Létrehozó:
PatakinĂŠ VĂĄrkonyi, Eszter
HorvĂĄth, Gabriella
SztĂĄn, Nikoletta
VÁradi, Éva
Barna, Judit
Kiadó:
AkadĂŠmiai KiadĂł
Dátum:
2012
info:eu-repo/date/embargoEnd/2032-12-31
Téma:
SV Veterinary science / ĂĄllatorvostudomĂĄny
Tartalmi leírás:
Although cryopreservation of avian semen is only applicable for singlegene traits, cryopreservation of avian blastodermal cells could facilitate preservation of the entire genome of endangered or rare-breed poultry. Slow freezing methods result in acceptable survival rates; however, there are apparently no reports regarding the use of vitrification. The aim of the study was to establish methods for chicken embryonic cell vitrification, including development of a container which supported cryopreservation of large numbers of cells (to increase the probability of chimera production). Based on a preliminary study, vitrification seemed to be practical for avian blastodermal cell preservation. Pieces of mosquito net as carrier increased live cell rates compared to pellet form in media containing two macromolecules. Furthermore, we concluded that fetal calf serum in the vitrification medium could be replaced by polyvinylpyrrolidone, a chemically defined substance free of unwanted growth factors and potential pathogens.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Patakiné Várkonyi, Eszter and Horváth, Gabriella and Sztán, Nikoletta and VÁradi, Éva and Barna, Judit (2012) Vitrification of early avian blastodermal cells with a new type of cryocontainer. Acta Veterinaria Hungarica, 60 (4). pp. 501-509. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess