Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA)
  • Metaadatok
Tartalom: http://real.mtak.hu/47529/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA)
Létrehozó:
Yan, HongBin
Bo, XinWen
Liu, Youyu
Lou, Zhongzi
Ni, XingWei
Shi, WanGui
Zhan, Fang
Ooi, HongKean
Jia, WanZhong
Kiadó:
Akadémiai Kiadó
Dátum:
2013
info:eu-repo/date/embargoEnd/2033-12-31
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Moniezia benedeni and M. expansa are common ruminant tapeworms of worldwide distribution, causing gastrointestinal disorders and even death in sheep and goats. In this study, a polymerase chain reaction- (PCR-) based approach for precise species identification was developed and also applied to faecal DNA diagnosis of the tapeworm infection. Since nuclear ribosomal DNA (rDNA) appears to be a useful target for species and/or strain markers, the 18S regions of the rDNA of M. benedeni and M. expansa were amplified and sequenced. The lengths and GC contents of the regions sequenced were 2476–2487 bp and 51.9–52.1% for M. benedeni and 2473 bp and 51.9–52.0% for M. expansa, respectively. Alignment and comparison of the 18S sequences of the two species showed 92.5–93.3% homology. No matches for the 18S regions of M. benedeni and M. expansa were found with other species by BLAST search, which made the 18S sequences appropriate markers for the design of distinctive primers for the two Moniezia species. Our 18S-based PCR could detect as low levels as 0.5 pg genomic DNA or the DNA extracted from 0.2 g faecal sample collected from the rectum of an M. expansa-infected goat. The results indicate that this PCR approach is a reliable alternative for the differential diagnosis of Moniezia species in faecal samples.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Yan, HongBin and Bo, XinWen and Liu, Youyu and Lou, Zhongzi and Ni, XingWei and Shi, WanGui and Zhan, Fang and Ooi, HongKean and Jia, WanZhong (2013) Differential diagnosis of Moniezia benedeni and M. expansa (Anoplocephalidae) by PCR using markers in small ribosomal DNA (18S rDNA). Acta Veterinaria Hungarica, 61 (4). pp. 463-472. ISSN 0236-6290
Kapcsolat:
Létrehozó:
info:eu-repo/semantics/embargoedAccess