The Active Enhancer Network Operated by Liganded RXR Supports Angiogenic Activity in Macrophages
  • Metaadatok
Tartalom: http://real.mtak.hu/46719/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Article
Cím:
The Active Enhancer Network Operated by Liganded RXR Supports Angiogenic Activity in Macrophages
Létrehozó:
Dániel, Bence
Nagy, Gergely
Hah, Nasun
Horvath, Attila
Czimmerer, Zsolt
Póliska, Szilárd
Bálint, Bálint László
Barta, Endre
Nagy, László
Dátum:
2014
Téma:
R850-854 Experimental medicine / kisérleti orvostudomány
Tartalmi leírás:
RXR signaling is predicted to have a major impact in macrophages, but neither the biological consequence nor the
genomic basis of its ligand activation is known. Comprehensive genome-wide studies were carried out to map
liganded RXR-mediated transcriptional changes, active binding sites, and cistromic interactions in the context of
the macrophage genome architecture. The macrophage RXR cistrome has 5200 genomic binding sites, which are
not impacted by ligand. Active enhancers are characterized by PU.1 binding, an increase of enhancer RNA, and
P300 recruitment. Using these features, 387 liganded RXR-bound enhancers were linked to 226 genes, which
predominantly reside in CTCF/cohesin-limited functional domains. These findings were molecularly validated
using chromosome conformation capture (3C) and 3C combined with sequencing (3C-seq), and we show that
selected long-range enhancers communicate with promoters via stable or RXR-induced loops and that some of the
enhancers interact with each other, forming an interchromosomal network. A set of angiogenic genes, including
Vegfa
, has liganded RXR-controlled enhancers and provides the macrophage with a novel inducible program.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Dániel, Bence and Nagy, Gergely and Hah, Nasun and Horvath, Attila and Czimmerer, Zsolt and Póliska, Szilárd and Bálint, Bálint László and Barta, Endre and Nagy, László (2014) The Active Enhancer Network Operated by Liganded RXR Supports Angiogenic Activity in Macrophages. GENES & DEVELOPMENT, 28 (14). pp. 1562-1577. ISSN 0890-9369
Kapcsolat:
MTMT:2702834; doi:10.1101/gad.242685.114