A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules
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Tartalom: http://real.mtak.hu/46204/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published



Type = Article
Cím:
A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules
Létrehozó:
Czimmerer, Zsolt
Hulvely, Julianna
Simándi, Zoltán
Várallyay, Éva
Havelda, Zoltán
Szabó , Erzsébet
Dezső, Balázs
Horváth, Attila
Török, Zsolt
Nagy, László
Bálint, Bálint László
Kiadó:
Public Library of Science (PLoS)
Dátum:
2013
Téma:
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
QH426 Genetics / genetika, örökléstan
Tartalmi leírás:
Short regulatory RNA-s have been identified as key regulators of gene expression in eukaryotes. They have been involved in the regulation of both physiological and pathological processes such as embryonal development, immunoregulation and cancer. One of their relevant characteristics is their high stability, which makes them excellent candidates for use as biomarkers. Their number is constantly increasing as next generation sequencing methods reveal more and more details of their synthesis. These novel findings aim for new detection methods for the individual short regulatory RNA-s in order to be able to confirm the primary data and characterize newly identified subtypes in different biological conditions. We have developed a flexible method to design RT-qPCR assays that are very sensitive and robust. The newly designed assays were tested extensively in samples from plant, mouse and even human formalin fixed paraffin embedded tissues. Moreover, we have shown that these assays are able to quantify endogenously generated shRNA molecules. The assay design method is freely available for anyone who wishes to use a robust and flexible system for the quantitative analysis of matured regulatory RNA-s.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Czimmerer, Zsolt and Hulvely, Julianna and Simándi, Zoltán and Várallyay, Éva and Havelda, Zoltán and Szabó , Erzsébet and Dezső, Balázs and Horváth, Attila and Török, Zsolt and Nagy, László and Bálint, Bálint László (2013) A versatile method to design stem-loop primer-based quantitative PCR assays for detecting small regulatory RNA molecules. PLOS ONE, 8 (1). e55168. ISSN 1932-6203
Kapcsolat:
MTMT:2276672; doi:10.1371/journal.pone.0055168