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A comparison of techniques for size measurement of nanoparticles in cell culture medium

  • Metaadatok
Tartalom: http://real.mtak.hu/45525/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
A comparison of techniques for size measurement of nanoparticles in cell culture medium
Létrehozó:
Gollwitzer, Christian
Bartczak, Dorota
Goenaga-Infante, Heidi
Kestens, V.
Krumrey, Michael
Varga, Zoltán
Kiadó:
Royal Society of Chemistry
Dátum:
2016
Téma:
Q1 Science (General) / természettudomány általában
Tartalmi leírás:
Plain and aminated silica nanoparticles dispersed in purified water, in 50 mM Tris-HCl buffer and in cell culture medium were measured using dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), small-angle X-ray scattering (SAXS), and particle tracking analysis (PTA). The test samples were measured by all methods immediately after dispersion and after incubation at room temperature for 24 h. The effect of the biological dispersion medium on the modal value of the particle size distribution was compared for each method taking into account the estimated uncertainty. For the methods based on light scattering, DLS and PTA, the size distributions obtained were significantly altered due to the formation of a protein corona and induced agglomeration effects. With SAXS and CLS, the measured size of the primary particles was mostly unchanged. While SAXS offers excellent precision and traceability to the SI unit system if the model fitting approach is used for data analysis, CLS provides detailed size distributions from which additional information on the agglomeration state can be deduced.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Gollwitzer, Christian and Bartczak, Dorota and Goenaga-Infante, Heidi and Kestens, V. and Krumrey, Michael and Varga, Zoltán (2016) A comparison of techniques for size measurement of nanoparticles in cell culture medium. ANALYTICAL METHODS: ADVANCING METHODS AND APPLICATIONS, 8 (26). pp. 5272-5282. ISSN 1759-9660
Kapcsolat:
MTMT:3109554; doi:10.1039/c6ay00419a