Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings

Metaadatok

Tartalom:	http://real.mtak.hu/44304/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings
Létrehozó:	KovÃ¡cs, BoglÃ¡rka PatkÃ³, DÃ¡niel SzÃ©kÃ¡cs, Inna OrgovÃ¡n, Norbert Kurunczi, SÃ¡ndor Sulyok, Attila Nguyen Quoc, KhÃ¡nh Vonderviszt, Ferenc HorvÃ¡th, RÃ³bert
Kiadó:	Elsevier
Dátum:	2016
Téma:	QH3011 Biochemistry / biokÃ©mia QH3020 Biophysics / biofizika
Tartalmi leírás:	Biomimetic coatings with cell-adhesion-regulating functionalities are intensively researched today. For example, cell-based biosensing for drug development, biomedical implants, and tissue engineering require that the surface adhesion of living cells is well controlled. Recently, we have shown that the bacterial flagellar protein, flagellin, adsorbs through its terminal segments to hydrophobic surfaces, forming an oriented monolayer and exposing its variable D3 domain to the solution. Here, we hypothesized that this nanostructured layer is highly cell-repellent since it mimics the surface of the flagellar filaments. Moreover, we proposed flagellin as a carrier molecule to display the cell-adhesive RGD (Arg-Gly-Asp) peptide sequence and induce cell adhesion on the coated surface. The D3 domain of flagellin was replaced with one or more RGD motifs linked by various oligopeptides modulating flexibility and accessibility of the inserted segment. The obtained flagellin variants were applied to create surface coatings inducing cell adhesion and spreading to different levels, while wild-type flagellin was shown to form a surface layer with strong anti-adhesive properties. As reference surfaces synthetic polymers were applied which have anti-adhesive (PLL-g-PEG poly(L-lysine)-graft-poly(ethylene glycol)) or adhesion inducing properties (RGD-functionalized PLL-g-PEG). Quantitative adhesion data was obtained by employing optical biochips and microscopy. Cell-adhesion-regulating coatings can be simply formed on hydrophobic surfaces by using the developed flagellin-based constructs. The developed novel RGD-displaying flagellin variants can be easily obtained by bacterial production and can serve as alternatives to create cell-adhesion-regulating biomimetic coatings. Statement of Significance In the present work, we show for the first time that - an oriented and dense monolayer of flagellin molecules mimics the surface of the bacterial flagellar filament. Consequently, the fabricated layer is completely cell repellent.- By genetically modifying flagellin, we incorporate cell adhesion regulating functionalities into this anti-adhesive coating.- We can easily tune the adhesion of living cells from completely cell repellent to highly adhesive. Â© 2016 Acta Materialia Inc.
Nyelv:	angol
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/44304/1/Kovacs_Flagellin_based_biomimet... KovÃ¡cs, BoglÃ¡rka and PatkÃ³, DÃ¡niel and SzÃ©kÃ¡cs, Inna and OrgovÃ¡n, Norbert and Kurunczi, SÃ¡ndor and Sulyok, Attila and Nguyen Quoc, KhÃ¡nh and Vonderviszt, Ferenc and HorvÃ¡th, RÃ³bert (2016) Flagellin based biomimetic coatings: From cell-repellent surfaces to highly adhesive coatings. ACTA BIOMATERIALIA, 42. pp. 66-76. ISSN 1742-7061
Kapcsolat:	http://real.mtak.hu/44304/ http://dx.doi.org/10.1016/j.actbio.2016.07.002 MTMT:3105834; doi:10.1016/j.actbio.2016.07.002