A novel method for assessing the chaperone activity of proteins
  • Metaadatok
Tartalom: http://real.mtak.hu/42289/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published

Type = Article
Cím:
A novel method for assessing the chaperone activity of proteins
Létrehozó:
Hristozova, Nevena
Tompa, PĂ©ter
Kovács, Dénes
Kiadó:
Public Library of Science (PLoS)
Dátum:
2016
Téma:
QH3011 Biochemistry / biokémia
Tartalmi leírás:
Protein chaperones are molecular machines which function both during homeostasis and stress conditions in all living organisms. Depending on their specific function, molecular chaperones are involved in a plethora of cellular processes by playing key roles in nascent protein chain folding, transport and quality control. Among stress protein families-molecules expressed during adverse conditions, infection, and diseases-chaperones are highly abundant. Their molecular functions range from stabilizing stress-susceptible molecules and membranes to assisting the refolding of stress-damaged proteins, thereby acting as protective barriers against cellular damage. Here we propose a novel technique to test and measure the capability for protective activity of known and putative chaperones in a semi-high throughput manner on a plate reader. The current state of the art does not allow the in vitro measurements of chaperone activity in a highly parallel manner with high accuracy or high reproducibility, thus we believe that the method we report will be of significant benefit in this direction. The use of this method may lead to a considerable increase in the number of experimentally verified proteins with such functions, and may also allow the dissection of their molecular mechanism for a better understanding of their function. © 2016 Hristozova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Hristozova, Nevena and Tompa, Péter and Kovács, Dénes (2016) A novel method for assessing the chaperone activity of proteins. PLOS ONE, 11 (8). e0161970. ISSN 1932-6203
Kapcsolat:
MTMT:3135639; doi:10.1371/journal.pone.0161970