Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells
  • Metaadatok
Tartalom: http://real.mtak.hu/40502/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells
Létrehozó:
Aekbote Lakshman Rao, Badri
Fekete, Tamás
Jacak, Jaroslaw
Vizsnyiczai, Gaszton
Ormos, Pál
Kelemen, Lóránd
Kiadó:
Optical Society of America (OSA)
Dátum:
2016
Téma:
QC02 Optics / fénytan
QH301 Biology / biolĂłgia
Tartalmi leírás:
We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015 Optical Society of America
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Aekbote Lakshman Rao, Badri and Fekete, Tamás and Jacak, Jaroslaw and Vizsnyiczai, Gaszton and Ormos, Pál and Kelemen, Lóránd (2016) Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells. BIOMEDICAL OPTICS EXPRESS, 7 (1). pp. 45-56. ISSN 2156-7085
Kapcsolat:
MTMT:3027725; doi:10.1364/BOE.7.000045