Diverging virulence types of intestinal and faecal isolates of porcine enteropathogenic Escherichia coli
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Type = Article
Cím:
Diverging virulence types of intestinal and faecal isolates of porcine enteropathogenic Escherichia coli
Létrehozó:
Malik, Anna
Nagy, BĂ©la
Kugler, Renáta
Szmolka, Ama
Kiadó:
Elsevier
Dátum:
2016-09
Téma:
QR Microbiology / mikrobiolĂłgia
Tartalmi leírás:
Enteropathogenic Escherichia coli (EPEC) frequently induce diarrhea in human infants and in young food producing
animals by causing attaching effacing (AE) lesions of the intestinal epithelium. Their pathogenicity has been proven
for rabbits, mice, lambs, and for colostrum deprived newborn pigs but not for conventional weaned pigs. Here we
aimed to compare in vivo pathogenetic potential and virulence genotypes of intestinal (n=6) and faecal (n=4) isolates of EPEC from weaned pigs. For this purpose we inoculated ligated ileal loops of 4 weeks old weaned pigs for assessment of EPEC adherence to ileal enterocytes by histology and immunohistology. We also determined the
virulence gene patterns of EPEC using an E. coli PCR-microarray. The intestinal EPEC isolates of sero-/intimin types O45:H11:eae-β, O49:NM:eae-β:, O84:H7:eae-γ, and O123:H11:eae-β formed adherent microcolonies of EPEC on
ileal villi more frequently (p<0.05) than faecal isolates of O28:H28:eae-NT, O108:H9:eae-β, O145:H28:eae-γ and
O157:H2:eae-β. Microarray and PCR analysis of both groups detected numerous virulence genes characteristic to
LEE (locus of enterocyte effacement), and non-LEE pathogenicity island of EPEC and enterohemorrhagic E. coli
(EHEC). Overall, intestinal isolates carried more (p<0.02) virulence genes than faecal isolates. Five of 6 intestinal
isolates contained the genes efal, lpfA, and tsh contributing to mucosal adhesion in contrast to the faecal isolates with none of these genes. Thus, the ileal loop model in weaned pigs is suitable to differentiate between intestinal and faecal isolates of porcine EPEC. Diverging virulence of the intestinal and faecal isolates is suggesting dynamic evolutionary events within the population of porcine EPEC.
Nyelv:
magyar
magyar
Típus:
Article
NonPeerReviewed
info:eu-repo/semantics/article
Formátum:
text
text
Azonosító:
Malik, Anna and Nagy, Béla and Kugler, Renáta and Szmolka, Ama (2016) Diverging virulence types of intestinal and faecal isolates of porcine enteropathogenic Escherichia coli. RESEARCH IN VETERINARY SCIENCE. ISSN 0034-5288 (Submitted)
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