Amphipathic helical ordering of the flagellar secretion signal of Salmonella flagellin
  • Metaadatok
Tartalom: http://real.mtak.hu/38685/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Amphipathic helical ordering of the flagellar secretion signal of Salmonella flagellin
Létrehozó:
TĹ‘ke, Orsolya
Vonderviszt, Ferenc
Kiadó:
Elsevier
Dátum:
2016
Téma:
QD04 Organic chemistry / szerves kémia
QH3011 Biochemistry / biokémia
Tartalmi leírás:
Export of external flagellar proteins requires a signal located within their N-terminal disordered part, however, these regions do not share any significant sequence similarity suggesting that the secondary/tertiary structure might be important for recognition by the export gate. NMR experiments were performed to reveal the conformational properties of the flagellin signal sequence in vitro. It assumed a largely disordered fluctuating structure in aqueous environment, but acquired a folded structure containing an amphipathic helical portion in 50% MeOH or upon addition of SDS micelles which are known to promote hydrophobic interactions. Our observations raise the possibility that the signal sequence may partially undergo amphipathic helical ordering upon interaction with the recognition unit of the flagellar export machinery in a similar way as revealed for protein import into intracellular eukaryotic organelles mediated by targeting signals of high diversity.
Nyelv:
angol
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
TĹ‘ke, Orsolya and Vonderviszt, Ferenc (2016) Amphipathic helical ordering of the flagellar secretion signal of Salmonella flagellin. Biochemical and Biophysical Research Communications, 476 (4). pp. 641-647. ISSN 0006-291X
Kapcsolat:
MTMT:3090960; doi:10.1016/j.bbrc.2016.06.012