Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells. (NDA@SZTAKI, Nemzeti Digitális Adattár, National Digital Data Archive)

Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells.

Metaadatok

Tartalom:	http://real.mtak.hu/34698/
Archívum:	MTA Könyvtár
Gyûjtemény:	Status = Published Type = Article
Cím:	Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells.
Létrehozó:	Fajka-Boja, Roberta SuhajdÃ¡nÃ© UrbÃ¡n, Veronika Szebeni, GÃ¡bor Czibula, Ãgnes BlaskÃ³, Andrea Kriston-PÃ¡l, Ã‰va Makra, IldikÃ³ Hornung, Ãkos SzabÃ³, EnikÅ‘ Than, NÃ¡ndor GÃ¡bor Monostori, Ã‰va
Kiadó:	Elsevier
Dátum:	2016
Téma:	QH3011 Biochemistry / biokÃ©mia QR180 Immunology / immunolÃ³gia RC658.5 Diabetes / diabetolÃ³gia
Tartalmi leírás:	BACKGROUND AIMS: Mesenchymal stromal cells (MSCs) have powerful immunosuppressive activity. This function of MSCs is attributed to plethora of the expressed immunosuppressive factors, such as galectin-1 (Gal-1), a pleiotropic lectin with robust anti-inflammatory effect. Nevertheless, whether Gal-1 renders or contributes to the immunosuppressive effect of MSCs has not been clearly established. Therefore, this question was the focus of a complex study. METHODS: MSCs were isolated from bone marrows of wild-type and Gal-1 knockout mice and their in vitro anti-proliferative and apoptosis-inducing effects on activated T cells were examined. The in vivo immunosuppressive activity was tested in murine models of type I diabetes and delayed-type hypersensitivity. RESULTS: Both Gal-1-expressing and -deficient MSCs inhibited T-cell proliferation. Inhibition of T-cell proliferation by MSCs was mediated by nitric oxide but not PD-L1 or Gal-1. In contrast, MSC-derived Gal-1 triggered apoptosis in activated T cells that were directly coupled to MSCs, representing a low proportion of the T-cell population. Furthermore, absence of Gal-1 in MSCs did not affect their in vivo immunosuppressive effect. CONCLUSIONS: These results serve as evidence that Gal-1 does not play a role in the systemic immunosuppressive effect of MSCs. However, a local contribution of Gal-1 to modulation of T-cell response by direct cell-to-cell interaction cannot be excluded. Notably, this study serves a good model to understand how the specificity of a pleiotropic protein depends on the type and localization of the producing effector cell and its target.
Nyelv:	angol
Típus:	Article PeerReviewed info:eu-repo/semantics/article
Formátum:	text
Azonosító:	http://real.mtak.hu/34698/1/Fajka_Boja2016Galectin_1_is_alo... Fajka-Boja, Roberta and SuhajdÃ¡nÃ© UrbÃ¡n, Veronika and Szebeni, GÃ¡bor and Czibula, Ãgnes and BlaskÃ³, Andrea and Kriston-PÃ¡l, Ã‰va and Makra, IldikÃ³ and Hornung, Ãkos and SzabÃ³, EnikÅ‘ and Than, NÃ¡ndor GÃ¡bor and Monostori, Ã‰va (2016) Galectin-1 is a local but not systemic immunomodulatory factor in mesenchymal stromal cells. CYTOTHERAPY, 18 (3). pp. 360-370. ISSN 1465-3249
Kapcsolat:	http://real.mtak.hu/34698/ https://dx.doi.org/10.1016/j.jcyt.2015.12.004 MTMT:3024122; doi:10.1016/j.jcyt.2015.12.004