Comparison of human and animal Bordetella bronchiseptica strains by PCR-RFLP and phylogenetic analysis
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Archívum: MTA Könyvtár
Gyűjtemény: Status = Published
Type = Conference or Workshop Item
Cím:
Comparison of human and animal Bordetella bronchiseptica strains by PCR-RFLP and phylogenetic analysis
Létrehozó:
Khayer, Bernadett
Magyar, Tibor
Wehmann, Enikő
Dátum:
2013
Téma:
SV Veterinary science / állatorvostudomány
Tartalmi leírás:
Bordetella bronchiseptica has a broad host range, it occurs in pigs (atrophic rhinitis), dogs (kennel cough) and cats (bordetellosis), and occasionally recovers from humans as well, especially from immunosuppressed patients. The classical Bordetella species (B. pertussis, B parapertussis and B. bronchiseptica) have evolved from a bronchiseptica-like ancestor, and the host range adaptation of B. bronchiseptica appears to be an ongoing process. This pathogen can adapt to the environment with virulence factors: toxins and surface elements (fimbriae, flagella). In this study, we characterised three regions (fimA, flaA and cyaA) of B. bronchiseptica strains originated from different hosts to reveal the diversity within the species by PCR-RFLP and to analyse the phylogenetic connections between strains. The examined strains were uniform by the fimA PCR-RFLP with HincII and SalI. The phylogenetic analysis resulted 0,0-2,9 percent divergence on nucleotide and deduced amino acid sequence level, and human strains were grouped into two separated branches. The flaA PCR-RFLP analysis by BglI and HincII divided the strains into eight groups. The percent divergence at nucleotide and amino acid level was 0,0-14,6 and 0,0-20,4. The human strains were grouped into three separated branches. The cyaA PCR-RFLP with NarI and SalI resulted four groups. The nucleotide percent divergence was 0,0-3,9, while the deduced amino acid divergence was 0,0-5,3. On the cyaA phylogenetic tree, the human strains were in four separated branches. The established divergence within human B. bronchiseptica strains and the diversity of the animal strains strengthened the zoonotic importance of this pathogen.
Founded by: OTKA K83332
Típus:
Conference or Workshop Item
PeerReviewed
Formátum:
text
Azonosító:
Khayer, Bernadett and Magyar, Tibor and Wehmann, Enikő (2013) Comparison of human and animal Bordetella bronchiseptica strains by PCR-RFLP and phylogenetic analysis. In: 10th International Symposium on Bordetella. Genomics and Evolution of Bordetellae session, 2013.09.08-2013.09.11, Dublin, Íroország.
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