| Tartalom: |
http://dx.doi.org/10.1016/j.jphotochem.2015.12.006 |
| Archívum: |
MTA Könyvtár |
| Gyűjtemény: |
Status = Published
Type = Article
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| Cím: |
New blue light-emitting isocyanobiphenyl based fluorophores: Their solvatochromic and biolabeling properties
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| Létrehozó: |
Nagy, Miklós
Rácz, Dávid
Kovács, Sándor Lajos
Lázár, László
Fehér, Péter Pál
Purgel, Mihály
Zsuga, Miklós
Kéki, Sándor
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| Dátum: |
2016
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| Téma: |
QD02 Physical chemistry / fizikai kémia
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| Tartalmi leírás: |
The preparation and optical study of 4-amino-4?-isocyanobiphenyl (ICAB) and its mono-and dialkylated derivatives (monoMICAB and diMICAB) are reported. They were found to be effective blue light emitters with a solvatochromic range of ??max = 104 nm, 92 nm and 90 nm, respectively. All three compounds turned out to be promising candidates in protein-labeling, yielding ?70-190× increase in their fluorescence intensity when mixed with 10× molar excess of bovine serum albumin (BSA). The binding constants for the BSA-fluorophore complexes were found to be KICAB-BSA = 3.8 × 104 ± 5.7 × 103 M-1, KmonoMICAB-BSA = 6.0 × 104 ± 3.9 × 103 M-1 and KdiMICAB-BSA = 2.3 × 104 ± 3.2 × 103 M-1. Time resolved fluorescence measurements revealed a nearly uniform ?2 ns lifetime for all three BSA-complexed ICAB derivatives. © 2015 Elsevier B.V. All rights reserved.
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| Típus: |
Article
PeerReviewed
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| Formátum: |
text
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| Azonosító: |
Nagy, Miklós and Rácz, Dávid and Kovács, Sándor Lajos and Lázár, László and Fehér, Péter Pál and Purgel, Mihály and Zsuga, Miklós and Kéki, Sándor (2016) New blue light-emitting isocyanobiphenyl based fluorophores: Their solvatochromic and biolabeling properties. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 318. pp. 124-134. ISSN 1010-6030
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