Metastasis-associated S100A4 is a specific amine donor and an activity-independent binding partner of transglutaminase-2.
  • Metaadatok
Tartalom: http://real.mtak.hu/32080/
Archívum: MTA Könyvtár
Gyűjtemény: Status = Published


Type = Article
Cím:
Metastasis-associated S100A4 is a specific amine donor and an activity-independent binding partner of transglutaminase-2.
Létrehozó:
Biri, Beáta
Kiss, Bence
Király, Róbert
Schlosser, Gitta (Vácziné)
Láng, Orsolya
Kőhidai, László
Fésűs, László
Nyitray, László
Kiadó:
Portland Press
Dátum:
2015-12-09
Téma:
QH3011 Biochemistry / biokémia
QH3015 Molecular biology / molekuláris biológia
Tartalmi leírás:
Transglutaminase-2 (TG2) is best known as a Ca(2+)-dependent cross-linking enzyme; however, some of its extracellular matrix-related functions are independent of its catalytic activity and include matrix remodelling, adhesion and migration. S100A4 belongs to the Ca(2+)-binding EF-hand S100 protein family and acts both intra- and extra-cellularly through binding to various partners. It regulates cell migration and its overexpression is strongly associated with metastasis and poor survival in various cancers. It has recently been suggested that TG2 mediates S100A4-dependent tumour cell migration. In the present study we provide evidence that S100A4 is an interacting partner and also a specific amine donor of TG2. TG2 incorporates a glutamine donor peptide to Lys(100) in the C-terminal random coil region of S100A4. Importantly, the enzyme activity is not necessary for the interaction: S100A4 also binds to TG2 in the presence of a specific inhibitor that keeps the enzyme in an open conformation, or to an enzymatically inactive mutant. We also found that S100A4 considerably enhances TG2-mediated adhesion of A431 epithelial carcinoma cells to the extracellular matrix. This role is independent of enzyme activity and requires the open conformation of TG2. We propose that S100A4 stabilizes the open conformation of TG2, which binds to its cell-surface receptor in this state and increases cell adhesion.
Nyelv:
magyar
Típus:
Article
PeerReviewed
info:eu-repo/semantics/article
Formátum:
text
Azonosító:
Biri, Beáta and Kiss, Bence and Király, Róbert and Schlosser, Gitta (Vácziné) and Láng, Orsolya and Kőhidai, László and Fésűs, László and Nyitray, László (2015) Metastasis-associated S100A4 is a specific amine donor and an activity-independent binding partner of transglutaminase-2. BIOCHEMICAL JOURNAL, 473 (1). pp. 31-42. ISSN 0264-6021
Kapcsolat:
10.1042/BJ20150843
TRANSPATH ITN 289964